Revealing <i>Candida glabrata</i> biofilm matrix proteome: global characterization and pH response

Candida glabrata is a clinically relevant human pathogen with the ability to form high recalcitrant biofilms that contribute establishment and persistence of infection. A defining trait auto-produced matrix, which suggested have structural, virulent protective roles. Thus, elucidation matrix components, their function modulation by host environment crucial disclose role in C. pathogenesis. As major step toward this end, study aimed reveal, for first time, proteome biofilms, characterize it bioinformatic tools its environmental pH (acidic neutral). The results showed presence several pH-specific proteins (51 acidic- 206 neutral-specific) also commonly found at both conditions (236). Of note, related mannan ?-glucan metabolism, potential delivery/organization carbohydrates were but much higher quantity under neutral environment. Additionally, virulence-related proteins, including epithelial adhesins, yapsins moonlighting enzymes, among proteins. Importantly, seem non-canonical secretion pathway Pdr1 was be regulator proteome. Overall, indicates impact cues provides unique resource further functional investigation contributing identification targets development new therapies against biofilms.